Introduction: The extraoral dry time and the storage media used to store teeth before reimplantation have the greatest impact on the prognosis of avulsed teeth. Aim: The purpose of this study was to evaluate and compare the efficacy of different storage media on viability of periodontal ligament cells at different time periods. Materials and Methods: Fifty freshly extracted sound teeth with healthy PDL were selected for the present study. Teeth were divided into four groups with 10 teeth each depending on the storage media (HBSS, COCONUT WATER, ALOE VERA, MILK) used for storing freshly extracted teeth. Remaining 10 samples were divided into 2 groups with 5 teeth each to serve as control. Cell viability in each group was checked at 1hr, 2hrs, 4 hrs, 8 hrs and 24 hrs time period. The data were tabulated and subjected to statistical analysis using One way Analysis of Variance (ANOVA) with post hoc analysis (Tukey HSD) for comparison of means. The statistical software namely SPSS 21.0 was used to analysis of the data. Results: The results indicated PDL cell viability was maximum at 1 hour time interval in all the groups and decreased over a period of time. Also, HBSS showed maximum percentage of viable PDL cells followed by Coconut water, Aloe Vera and milk at all time intervals tested. Conclusion: It can be concluded that HBSS is the gold standard for the preservation of viable cells for a longer period followed by Coconut water and Aloe Vera.