Background: Irrigants and intracanal medicaments form a necessary adjunct that enhances the antimicrobial effect of mechanical cleansing and thus augments overall clinical efficacy. Hence; the present study was undertaken for analyzing and comparing the efficacy of three endodontic irrigating solutions against Enterococcus Faecalis. Materials & methods: A total of thirty single rooted mandibular first premolar specimens were included. Sectioning of the specimens was done at cement-enamel junction level. Instrumentation of the root canals was done with rotary instruments to an apical size of ProTaper F3. Vertical sectioning of all the specimens was done along the mid-sagittal plane into two halves. All the specimens were divided into following study groups: Group A: 5% Sodium hypochlorite, Group B: 0.2% Chitosan and Group C: Saline. Inoculation of the bacterium was done in the broth in all the tissue culture wells. The biofilm on root canal surface was taken with a sterile scalpel and inoculated on agar plates and incubated. Colony forming units were then analyzed. Analysis of all the results was done using SPSS software. Results: Mean CFU among specimens of group A, group B and group C were found to be 0.59, 2.118 and 90.184 respectively. While analyzing the results statistically, significant results were obtained. Conclusion: Sodium hypochlorite exhibited maximum efficacy against Enterococcus Faecalis.