Urinary tract infection (UTI) is a group of diseases that arise from bacterial colonization of the genitourinary tract, which spans from the kidney's renal cortex to the urethral meatus. A total of 90 individuals with UTI were noticed in the current investigation. There were 45 diabetes individuals and 45 non-diabetic people among them. In this present study, the diabetes group included 26 females and 19 males, whereas the non-diabetic group possessed 29 females and 16 men. There was an important variation in the subjects' urine sugar levels. Rising blood sugar levels were elevated in 45.55% of patients, while postprandial blood sugar levels were elevated in 31.11% of cases. To analyze the study's major observations, several laboratory testing and resistance to medication tests were done.SPSS was employed to verify the study's findings. E Coli was the most often identified pathogen in both diabetes and non-diabetic individuals (46.7% and 53.3%, respectively). Klebsiella spp. came in second for both those with diabetes and non-diabetics, contributing to 61.5% and 38.5% of cases, respectively. The comparison of the number of medications resistant in diabetes and non-diabetic groups was shown to be significant, with the diabetic group having a higher total number of drug obstruction.