Introduction: Catheter-related bloodstream infection (CRBSI) is a critical problem in dialysis center. Aim: To study the prevalence of central venous catheter related blood stream infections and to identify the microbiological profile of organisms causing CRBSI. Materials and Methods: This prospective observational study carried out at Dialysis unit over a period of two years on 145 hemodialysis patients, who had cultures of catheter and blood samples were studied. Results: A total of 557 haemodialysis catheters (tunnelled and non-tunnelled) were inserted during the study period, and among them, CRBSIs was seen in 145 cases.Mean duration of dialysis catheters in situ was 20.6days.99 (68.2%) patients were males, and 46 (31.7%) were females. Diabetes mellitus (76.5 %) was the most common premorbid illness associated with CRBSI. 81.3% patients found to be below 10 mg/dl of Hb, and 68.3%patients had leucocytosis. Among 145 of CRBSI, blood cultures were positive in 48 patients, and 97 patients were culture negative.45.8% isolates were gram-positive and 52.8% isolates were gram-negative bacteria, and one isolate was found to be fungal infection.Among gram positive, CONS were most isolated, followed by Enterococcus, MSSA and MRSA.