Introduction:Obesity is a health and dietary problem in both developed and developing countries, Obese people have a higher risk for type 2 diabetes mellitus, cardiovascular, hypertensive, dyslipidaemia and cancer. This would also increase the frequency of oxidative stress due to the difference in the number of oxidants and antioxidants, which contributes to unpaired mitochondria and β oxidation, resulting in increased reactive oxygen species.Antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GSH), and non- antioxidants such as vitamin C, E act as free scavengers, therebyreducing oxidative stress. Thus, we aimed to study Antioxidant levels in this study mainly, Superoxide dismutase, Glutathione peroxidase, Vitamin C, and Vitamin E. Methods: This cross-sectional study was carried out on 100 Obese patients and 100 healthy controls and thelevels of Superoxide dismutase, Glutathione peroxidase, Vitamin C, and Vitamin E are measured before and after giving supplements. Results:In obese patients Superoxide dismutase (SOD) levels were 142.15±21.31, Glutathione Peroxidase levels were 1.941±2.36, Vitamin C levels were 0.29±0.08 and Vitamin E levels were 3.906±1.31 which is lower than normal range. Conclusion:Present study concludes that estimation of antioxidant capacity is used as one of the biological markers for monitoring oxidative stress in obese persons.