Background &Method: Present study was conducted with an aim tostudy clinical and ultrasonography evaluation of pregnancy of unknown location in first trimester of pregnancy. 500 Pregnant patients in the first trimester of pregnancy were included in the study. Complete clinical evaluation and general physical examination were performed and patients were then subjected to ultrasound examination. Result:Among 500 pregnant females almost 5% were pregnancy of unknown location. 4% of all the pregnancy of unknown location was diagnosed to be ectopic pregnancy. 0.6% of pregnancy of unknown location was failed PUL. 0.4% of pregnancy of unknown location was persistent PUL. β-hCG level is best indicator for predicting ectopic pregnancy while progesterone is best indicator for viability. Serum progesterone levels can be helpful at determining the viability of pregnancies; however, they are not specific to pregnancy location. Conclusion:Ultrasonography is paramount in utility as a diagnostic tool for ANC patients. When used in combination with clinical information and serum β-hCG levels, it can provide diagnostic and prognostic information regarding pregnancy confirmation, viability and rapid information regarding life-threatening conditions. One great concern of PULs is that they are cases of ectopic pregnancy whose diagnosis might be postponed.