Background: Chronic dacryocystitis is the most prevalent adnexal illness encountered in routine treatment and is a major cause of ocular morbidity in India. Methodology: This cross-sectional study comprised 200 consecutive individuals with persistent dacryocystitis. All subjects had baseline examination after providing signed informed consent. By applying pressure to the lacrimal sac and allowing the regurgitant material to reflux via the lacrimal puncta, or by irrigating the lacrimal drainage system with sterile saline and collecting the sample from the refluxing material, sample fluid was collected. Gram staining and culture were performed on the samples, which were delivered to the microbiology department. The Kirby Baur disc diffusion test was used to assess antibiotic sensitivity in cultured bacterial growth. Results: Chronic dacryocystitis is most common in the fifth decade of life. Females are more likely than males to develop chronic dacryocystitis. The left eye was more affected than the right. The majority of patients developed mucopurulent discharge. When compared to cases with serous discharge, the percentage of samples yielding positive growth on culture was higher in cases with mucopurulent and purulent discharge. On culture, the majority of patients with serous discharge exhibited no growth. The majority of individuals had epiphora with mucopurulent or purulent discharge. The most common nasal association was a deviated nasal septum. Microorganisms were found in 58.7% of the samples cultivated. Conclusion: Knowledge of the bacteriology of chronic dacryocystitis aids in the selection of prophylactic antimicrobial drugs that act precisely on the pathogenic organism and also helps to prevent antibiotic resistance induced by injudicious antibiotic usage.