Backgrounds and objectives: Urinary Tract Infection (UTI) is an infection of one or more structures in the urinary system. The urinary tract includes urethra, bladder, ureters, prostate and kidneys. Clinical presentation of UTI varies from asymptomatic infection to full blown pyelonephritis. Some symptoms may be non-specific and frequently symptoms overlap in patients with lower UTIs and upper UTIs. Present study was undertaken to isolate and characterize pathogens causing UTIs in patients attending, DMCH Darbhanga, a rural tertiary care hospital and to study the antimicrobial sensitivity pattern of the urinary isolates, so as to provide a basic guideline in treating UTIs. Methodology: A total of 500 midstream urine (MSU) samples were taken from both male and female patients who had clinically suspected UTI .They were asked to collect a fresh sample of MSU in a sterile container after cleaning the genitals with soap and water. The sample was transported to the microbiology laboratory and processed by wet film microscopy, Gram’s stain and semiquantitative culture in blood agar and MacConkey’s agar .All the isolates were identified by standard procedures and antimicrobial susceptibility test was done for the bacterial isolates. Conclusion: The most common uropathogen isolated in our study was E. coli. Pseudomonas aeruginosa was the second major pathogen isolated from UTI. In the present study, overall incidence of UTI observed among males is marginally higher than that was observed in females. High rate of UTI was observedin children of 0 – 9 years of age.