Cardiovascular diseases (CVDs) comprise disorders of the heart and blood vessels and still represent a major cause of death globally. CVDs have been shown to be responsible for approximately 17.9 million deaths each year, which accounts for 31% of all deaths worldwide. Among the various CVDs, myocardial infarction (MI) is a major cause of mortality and morbidity across the world. The different doses of Boswellia serrata (100, 200, and 400 mg/kg) and metoprolol (10 mg/kg) were calculated based on the animal’s body weight and were administered per oral for 30 days. Parameter for assessment of the effect of Boswellia serrata isoproterenol-induced cardiomyopathy in rats: Body weight, Electrocardiographic, Hemodynamic, Heart Weight, Heart/Body Weight, CKMB, LDH, ALP, Anti-oxidant activity (Total protein, MDA, nitric oxide, GSH and SOD) in cardiac tissue, Histopathology of cardiac tissue. treatment with metoprolol (10 mg/kg) significantly (P < 0.001) decreased RR interval as compared to vehicle control rats. Treatment with BS (100 and 200 mg/kg) showed a significant and dose-dependent (P < 0.01 and P < 0.001) decreased in RR interval as compared to vehicle control rats.