The main objective of this study is to offer a rapid and efficient green production of silver nanoparticles with known antibacterial properties. In the current study, a biological approach was applied at room temperature to biosynthesize silver nanoparticles from Chrysanthemum moriflorum flower extract. Aqueous floral extract from Chrysanthemum moriflorum was used to make AgNPs from silver ions fast. This process became apparent when silver nitrate solution was added to the bright yellow flower extract solution, which caused it to turn brown. The biosynthesized AgNPs were examined using transmission electron microscopy (TEM) and ultraviolet-visible (UV-Vis) spectroscopy (TEM). The UV spectra revealed AgNPs' distinctive surface plasmon resonance band at 431 nm. The generated AgNPs, a powerful broad spectrum antibacterial agent, successfully inhibited Escherichia coli and Bacillus sp. To combat the rise in antibiotic resistance, AgNPs made from environmentally acceptable ingredients like flower extract offer a potential weapon.