Fluoride the anionic form of fluorine is widely distributed in nature. The number of Fluorosis afflicted countries has been steadily increasing. The problem of high fluoride content in drinking water resources leads consequently a higher incidence of fluorosis. In addition to conventional methods, an attempt of Biosorption was done in this research work for effective removal of Fluoride from Ground waters. The physicochemical analysis of groundwater samples were carried out to identify the fluoride rich water bodies. A total of 200 bacterial strains were isolated from soil sample of Baratang Island, Andaman and were subjected to primary screening where 16 strains were showed good absorption. The effects of nutrients, physical factors were studied with these strains and finally a total of 5 strains were identified as best absorbers and subjected field sample analysis. The experimental results indicate that the identified bacterial strains have reduced more than 50% of the initial concentration of fluoride in all the studied conditions and they may be considered for Defluoridation of drinking water after careful evaluation of the methodology under various field conditions.