Abstract: According to recent World Health Organization reports, Tuberculosis is regarded to be the most severe disease caused by infection. There are significant drawbacks to the testing used for TB diagnosis and there is no accurate point-of-care (POC) diagnostic examination. New methods are required worldwide to controlling tuberculosis (TB). In general, new diagnostic methods and new biomarkers are necessary to assess both pathogenic and host main elements of an infection response. Biomarker-based or multiple marker biosignature-based tests, preferably conducted on blood or urine, for the identification of active TB may help achieve the target drug profiles recommended for point-of-care testing by the World Health Organization. Here I review The identified forms of biomarkers included antibodies, cytokines, metabolic markers, mycobacterial antigens and volatile organic compounds and the different biomarkers of tuberculosis, like,LAM ,IFN-g ,myolic acid ,antigen 85,different VOCs,Rv1681 protein,CFP-10,granzyme A