Infertility is a disease of the reproductive system and its treatment can affect all aspects of people’s lives, which can cause various psychological-emotional disorders or consequences including frustration, hopelessness, depression, guilt, anxiety and feelings of worthlessness in life. In order to evaluate Biochemical and endocrinological aspects of female infertility, a test-control study was designed. For the study 150 clinically diagnosed infertile female subjects and 150 age matched healthy females with one or more children were involved in the study as control. Level of uric acid concentration was comparatively higher among test group (5.5mg/dL) than the control subjects (4.4mg/dL) (p<0.005). The inflammatory marker (hsCRP) demonstrated a very high concentration among the test subjects than the control group (1.0±0.59) (p<0.005). It was evident that, study group expressed an FSH concentration of 30±10 and control with a FSH level of 12.8±6.00 (p=<0.005).