Detection of biofilm, slime and motility patterns in Pseudomonas spp. can be valuable in empathetic the virulence of the creature. Here studies on the phenotypic methods of biofilm production, slime production and the motility patterns exhibited in 69 clinical isolates of Pseudomonas spp. and 2 environmental isolates were performed. Nineteen strains produced biofilm in which 6 were from HIV and 13 were from Non-HIV patients. All the 19 isolates positive for biofilm production were taken for detection of biofilm genes by PCR. The gene coding for alkaline protease aprA gene was detected in 13 (68.42%) isolates. The gene for phenazine biosynthesis phzA1 gene was detected in 14 isolates. The house keeping gene acpP was found to be positive for 18 (94.7%) isolates. 48 isolates were found positive for slime production. Pellicle formation was positive in 39 isolates. Swarming motility was positive in 42 isolates 29 were from HIV and 11 were from Non-HIV. Swimming motility was exhibited in 31 isolates. Twitching motility was exhibited in 35 isolates. The 2 environmental isolates were found to be positive only for slime production, swarming, and swimming motility. Among the clinical isolates, HIV isolates showed high production of slime, pellicle and positivity for the motility patterns seemed to be high in HIV compared to Non-HIV isolates except for the biofilm production which is high in Non-HIV isolates.