Samples were collected from river mud close to the sediments of sewage estuaries that flow directly to the main river for most areas of Anbar Governorate, which starts from Al-Qaim district and is considered the beginning of the mouth of the Euphrates River from western Iraq to Fallujah in the east, with 9 regions (36 samples), which are (Al-Qaim - Al-Obaidi - Rawah - Anah - Haditha - Al-Baghdadi - Hit - Ramadi - Fallujah) over a range of about 400 km2, where samples were taken from October 2019 until June 2020 in four seasons (Fall - Winter - Spring – Summer).for site study of the phytoplankton community and its impact on the river water quality, comparing the characteristics in one season and then the four seasons. The current study showed that the highest number of Bacillus bacteria was in the summer season. Several other genera such as E. coli, Klebsiella, Pseudomonas and some other pollutants were isolated. The highest percentage of bacterial contaminants was also recorded after the estuary compared with other sites.A 73 species of algae were diagnosed in different regions of Anbar Governorate, and the results showed that there were no significant differences between the different seasons of the year with regard to algae, and the current study also demonstrated the presence of significant differences in most algae after the downstream compared to the rest of the sites.