Background- Thyroid hormones play important role in normal growth and maturation of skeletal system. Thyroid function regulates a wide array of metabolic parameters. Thyroid gland produce two important hormones triiodothyronine (T3) and thyroxine (T4) which are known as T3 and T4. Hypothyroidism and hyperthyroidism are two primary pathological conditions that involve thyroid glands. Disorder of thyroid gland are among the most abundant endocrine disorders in India Aims and Objectives-To investigate the association of Lipid profile in thyroid dysfunction patients. Materials And Methods- A total of 100 thyroid dysfunction patients visiting the outpatient Department of General Medicine of Mahatma Gandhi Medical College & Hospital, Jaipur was enrolled for the study. Age up to 60 years, either gender and Patients who were willing to participate and sign consent document. Blood samples after overnight fasting were collected by standard aseptic techniques. The samples collected were subjected to following investigations: Serum T3, T4, TSH and Serum Lipid profile (Serum cholesterol, Triglycerides, HDL cholesterol, LDL cholesterol, & VLDL). Results- In this study the mean serum T3, T4 and TSH levels among the three groups. Mean Serum T3 levels were reported low as 0.89 ng/ml in the hypothyroid group and as high as 2.13 ng/ml in the hyperthyroid group. The mean T4 levels were reported low as 3.22 µg/dl in the hypothyroid group while it was as high as 17.39 µg/dl in the hyperthyroid group. Mean serum TSH was reported 68.93 µIU/ml in hypothyroid group and 0.22 µIU/ml in hyperthyroid group