ABSTRACT The case-control study was aimed to evaluate the effect of IL-4 and IL-6 gene polymorphism on susceptibility to pulmonary tuberculosis in patients in Babylon province and also measure the serum level of these cytokine in in sera of studied group. The genotype association of rs2243250 (C/C, C/T, T/T) were significant (P<0.05) with (46.7, 38.3, 15)% respectively for PTB cases and (36.7, 26.6, 36.7)% respectively for control group. Allele's frequency for patients and control for IL-4 (rs2243250) were also significant (P<0.05) and the C allele represent the risk allele for PTB patients with odd ratio (95% CI) of 1.93(1.15-3.24), while the T allele represent protective allele for control group 0.52 (0.31-0.87). The genotype association of rs1800796 (C/C, G/C, G/G) were significant (P<0.05) with (43.3, 38.3, 18.4)% respectively for PTB cases and (50, 15, 35)% respectively for control group, whereas, the allele's frequency for patients and control of IL-6 (rs1800796) were insignificant with (P>0.05). There was a significant difference in mean level of both IL-4 and IL-6 serum concentration between pTB patients and controls (P<0.01). Also, there were an insignificant effect of IL-4 (rs2243250) and IL-6 (rs1800796) gene polymorphism on the difference in serum level of both IL-4 and IL-6 respectively in studied groups (P>0.05).