Background: Hypersegmentation of neutrophils is defined as presence of 5% or more neutrophils with five or more lobes or single neutrophil with 6 lobes. It is usually associated with deficiency of or failure to utilize cobalamin or folate and impaired DNA synthesis. Aims & Objectives: to find out any abnormality in the neutrophils of those subjects having hypersegmented neutrophils, to assess the manifestations in peripheral smear of these patients and to find out any association of the presence of hyper segmented neutrophils in peripheral smear with Vitamin B12 level,