Saudi Arabia is considered to be an area of endemic hepatitis B virus (HBV) infection. Among Saudi children, the overall HbsAg carrier rate dropped from 6.7% in 1989 to 0.3% in 1997 after mass HB vaccination program among adults prevalence is 0.22%. The prevalence varied by region, ranging from 0.03% to 0.72% with a mean prevalence of 0.15%.The coverage of hepatitis B vaccination remains low in developing countries to date. Building capacity in hepatitis B virus prevention and management for students is one of the pillars of the national viral hepatitis control strategy. The hepatitis B virus (HBV) is considered a global problem which threatens the public health It may cause chronic infection which develops to liver cirrhosis and leads to death. The population infected with HBV has been found to be about 2 billion people, whereas annually 360 million people getting chronic liver disease and 600 thousand facing death either from chronic liver disease or liver cancer globally. Aim of the study: To assessment the knowledge among male School teachers about Hepatitis B at Makkah Al Mukarramah in Saudi Arabia 2021 Methods: A Cross sectional community based study was adopted. It included all male school teachers in primary, intermediate and secondary stages at primary health care center in Makkah Al Mukarramah. A self-administered valid questionnaire was utilized for data collection. It included personal data, questions regarding etiology, epidemiology, symptomatology, risk factors, preventive measures and outcome of hepatitis B. Results: Conclusion: inadequacy of knowledge regarding HBV among teachers sector, Makkah in certain key areas of HBV. This lack of knowledge is a matter of concern since teachers could disseminate their information to young youth at schools. Exposure to information , support from institutions, and financial support related to vaccination cost have a positive impact on the knowledge about hepatitis B infection and vaccination coverage.