Outbreak of pandemic SARS-CoV2 in 2020 collapse the healthcare management system all around the world. People were scared, as invisible virus spreading its infection among the individual who were close contact with infected person via droplet nuclei or aerosol transmission. The only gold standard test for early detection of viral genome that time was RT-PCR tests which was due to its high sensitivity and specificity nature. During Covid period RT-PCR sensitivity was reported much less all around the world. Hence there is urgent need to find out the reason for less sensitivity or fallacy report of RT-PCR or developed alternative method for SARS-CoV-2 and its strain detection method in less time with High efficacy.