Background: Overcoming the scourge of malaria infection is possible when healthcare workers have good levels of understanding and attitudes towards the disease, which will influence their treatment practices. Saudi Arabia and Yemen are the only two countries in the Arabian Peninsula that are yet to achieve malaria elimination. This study assessed Knowledge of Malaria fever causes and symptoms among health care workers at the primary healthcare in Makkah City at Saudi Arabia. Malaria is one of the deadliest mosquito-borne diseases in the world. More than 80% of the total populations are at risk of malaria in the 22 countries in Asia and the Pacific. South Asia alone is home to an estimated 1.4 billion people at risk of contracting malaria. Aim of the study: The study aimed to assess the knowledge about Malaria fever causes, signs and symptoms among health care workers at the primary healthcare in Makkah City at Saudi Arabia. Methods: Across sectional descriptive study conducted among including health care workers at the primary healthcare in Makkah Al-Mokarramah city, during the October to December, 2020, the Sample size of medical practitioners. Our total participants were (350). Results: shows the Total knowledge of the malaria fever results show the majority of participant had weak information were(62.6%) while average of the Knowledge about participants signs of the malaria fever were(33.4%) the data ranged from(1-24) by mean ±SD(11.094±4.503) and a statistical significant relation While Chi-square X2 180.109 and P=value 0.001and that is a significant positive correlation between Knowledge about causes and signs were r= 0.70) and p-value =0.001 Conclusion: Malaria remains a public health problem in most governorates of KSA region. Health professionals have suboptimal knowledge and practice levels regarding causes, signs, symptoms, standard precautions of infection control, while most of them have positive attitude. The identification and monitoring of malaria transmission hotspots and predictors would enable control efforts to be intensified and focused on specific areas and therefore expedite the elimination of residual malaria from the whole region Therefore, it is recommended to enforce their training