Background: Chronic Kidney Disease (CKD) is a progressive loss of kidney function and is a worldwide public health problem both for the number of patients and for the cost of treatment. Trace elements such as copper (Cu) is altered in CKD. We assessed 100 subjects of both sex with different age groups, among them 50 are normal healthy controls (group 1) and 50 are CKD patients (group 2) from dialysis ward (medicine) S.R.G. Hospital, Jhalawar Medical College, Jhalawar (Raj.) Serum copper was estimated using Flame Atomic Absorption Spectrophotometer (AAS).