INTRODUCTION: Diabetes mellitus is one of the most common health problems facing health care professionals. Chronic hyperglycemia is commonly associated with long term dysfunction of various organs including the nervous system. As the duration of diabetes increase, diabetic patients develops various microvascular (like neuropathy, nephropathy and retinopathy)complications.Therefore this study was intended to determine the nerve conduction velocity (NCV) of male diabetic offspring in Eastern Uttar Pradesh. MATERIAL AND METHODS: This study was conducted in the Department of Physiology, MahamayaRajkiya Allopathic Medical College Ambedkar Nagar,Uttar Pradesh. Median nerve is one of the major peripheral nerve was selected for NCV. NCV of all the subjects were done by NeuropackX1(Nihon Kohden). RESULT:The median motor nerve conduction velocity (59.52 ± 2.98) and sensory nerve conduction velocity (56.58 ± 2.73) of diabetic offspring was insignificantly (p>0.05) decreased as compared to the median motor nerve conduction velocity (59.66 ± 3.24) and sensory nerve conduction velocity (56. 84 ± 2.79) of non-diabetic offspring.In addition,The median motor nerve conduction velocity (62.31 ± 2.23) and sensory nerve conduction velocity (54.77 ± 2.70) of diabetic male offspring was insignificantly (p>0.05) decreased as compared to the median motor nerve conduction velocity (63.72 ± 1.24) and sensory nerve conduction velocity (54. 81± 2.65) of non-diabetic male offspring.Furthermore,The median motor nerve conduction velocity (62.01 ± 2.23) and sensory nerve conduction velocity (55.19 ± 2.70) of diabetic female offspring was insignificant (p>0.05) decreased as compared to the median motor nerve conduction velocity (63.3 ± 1.24) and sensory nerve conduction velocity (54. 4± 2.60) of non-diabetic female offspring.