Biomedical waste (BMW) management is an issue of major concern of all healthcare providers and healthcare establishments as the waste produced during the course of healthcare activities carries potential for infection and injury than any other type of waste. Biomedical waste management has recently emerged as an issue of present concern not only to hospitals, nursing home authorities but also to the environment. The bio-medical wastes generated from health care units depend upon a number of factors such as waste management methods, type of health care units, occupancy of healthcare units, specialization of healthcare units, ratio of reusable items in use, availability of infrastructure and resources etc. The proper management of biomedical waste has become a worldwide humanitarian topic today. Many studies across the country have shown that there are still deficiencies in the Knowledge, attitude and practices of the health care professionals in the organizations. Hence, A cross sectional study was carried out to assess the knowledge, attitude and practices regarding biomedical waste management among nursing professionals in a tertiary care hospital Bhubaneswar, Odisha. With due consideration to nursing personnel’s availability, accessibility, feasibility, and resources, we decided to include (n=100) of them in our study. The number of nursing personnel was selected by simple random sampling in their respective department/specialty. A pretested self-administered questionnaire was used to assess the knowledge, practices and attitude of the nursing personnel regarding biomedical waste management. Overall, the knowledge, attitudes and practices towards biomedical waste management among the nursing professionals were satisfactory. Complete hepatitis B vaccine coverage among nurses (100%) in our study is attributed to hospital policy of free immunization to high risk groups. Such policy decisions do have a positive impact on work efficiency of nurses by creating a sense of being protected.