Abstract: Background: Oral and maxillofacial imaging has been long an indispensable component while diagnosing in dentistry. The biologically hazardous or adverse effects on human body are well documented. This warrants judicious use to avoid its harmful effects. Manpower assisting dental practitioners in routine private dental practice in India is an unmapped manpower. Aim: This is a maiden attempt to map manpower assisting dental practitioners in private dental clinics along with assessment of their knowledge, attitude and adherence to radiation safety measures and radiological waste management in Wardha. Methodology: This cross-sectional study will utilize 26 point structured; validated and pre-tested questionnaire regarding knowledge, attitude and adherence to radiation safety measures and radiological waste management. Statistical analysis: The data collected will be computed and chi square test, unpaired t test, one way ANOVA followed by post hoc test and Pearson’s correlation coefficient will be used appropriately for statistical analysis. Implications: At the end of study, mapping of manpower assisting dental practitioners will be done. The degree of knowledge, attitude and adherence to radiation safety measures and radiological waste management among manpower assisting dental practitioners will be obtained and the findings can be shared with apex council for the designing and implementation of formal training program for these dental assistants.