Background: World is facing the most serious problem that is population explosion. Contraception method is used worldwide for over birth control or unwanted pregnancies. Hence; the present study was undertaken for assessing the impact of oral contraceptives on lipid profile in Premenopausal Women. Materials & methods: A total of 30 pre-menopausal subjects were enrolled. All the subjects belonged to the age group of 30 to 40 years. Only those subjects were included in the study group which has been using oral contraceptives for the last 3 months. Another set of 30 subjects with negative history of intake of oral contraceptives were taken as controls. Complete demographic and clinico details of all the patients were obtained. Blood samples of all the patients were obtained and were sent for analysis of lipid profile. All the results were recorded in Microsoft excel sheet and were analysed by SPSS software. Results: Mean serum cholesterol levels among the patients of the study group and the control group was 196.5 mg% and 181.4 mg% respectively. Mean serum triglyceride levels among the patients of the study group and the control group was 95.4 mg% and 82.6 mg% respectively. Mean serum high density lipoproteins levels among the patients of the study group and the control group was 41.6 mg% and 42.8 mg% respectively. Mean serum cholesterol levels and serum triglyceride levels among the patients the study group was significantly higher in comparison to the patients of the control group. Conclusion: Use of oral contraceptives significantly alters the lipid profile in premenopausal women.