Introduction: OPMDs are oral mucosal lesions with an increased peril for malignant transformation, most common being leukoplakia. The chief inflammatory cells accounting for transferring host response to malignancy includes macrophages, plasma cells, neutrophils, eosinophils and mast cells. Both eosinophils and mast cells modulate their function by the release of certain proinflammatory mediators. These bidirectional interactions between the mast cell and eosinophil cell might play an imperative part in the pathogenesis and progression of disorders. Intention of the research was to qualitatively and quantitatively evaluate eosinophil cells and mast cells in leukoplakia cases and control cases histochemically using congo red stain and toluidine blue stain respectively.