ABSTRACT: Background: The present study was conducted to assess collagenase activity and MMP levels in GCF and in peri- implant sulcular fluid in healthy, chronic periodontitis and peri- implantitis patients. Materials & Methods:40 patients of both genders were divided into 4 groups. Group I patients were healthy, group II had healthy dental implants, group III patients had periodontitis and group IV had peri-implantitis. Collagenase activity wasmeasured using a DNP-synthetic octapeptide. Results: The mean gingival index was 0.6 in group I, 1.1 in group II, 2.2 in group III and 2.3 in group IV. Probing depth was 3.2 in group I, 2.5 in group II, 5.7 in group III and 5.1 in group IV. Collagenase activity per site was 1.7, 0.5, 15.2 and 14.7 in all groups respectively. Collagenase activity per ml was 9.2, 2.2, 16.4 and 21.6 in all groups respectively. Conclusion: Peri-implantitis PISF contained higher collagenase-2 levels. GCF and PISF from severeCP and PI exhibited the highest activation of MMP-8 isoenzymes species.