Background: Febrile neutropenia is a medical emergency. The present study was conducted to assess clinical and microbiological profile of pathogens in febrile neutropenia in haematological malignancies. Materials & Methods: 70 patients of haematological malignancies who developed neutropenia (<500/mm3) and fever ≥ 1000 F were selected. Microbiological culture of urine and blood was carried out. Clinical profile and sensitivity pattern of isolated microorganisms was recorded. Results: Out of 70 patients, males were 40 and females were 30. Clinical features were fever in 56, GI symptoms in 34, bleeding in 15, vertigo in 2, infection in 7, splenomegaly in 5 and hepatomegaly in 17. The difference was significant (P< 0.05). Haematological disorders found to be ALL in 20, AML in 15, aplastic anemia in 10, lymphoma in 6, CML in 4, APML in 3 and other in 2 patients. Gram positive organism found were MRSA in 2, MSSA in 3, Strep. spp. In 4, and enterococcus spp.in 3 cases. Gram negative organism found were E. coli in 24, K. pneumoniae in 16, Klebsiella spp.in 8, pseudomonas spp.in 6 and P. aeruginosa in 2 cases. In maximum cases antibiotic sensitivity was found against PIP/TAZO, CEFO/SAL and CEFTAZI.