Background: Urinary stone disease (USD) is an increasing clinical problem in both children and adults. The present study was conducted to assess bacteriological profile of bladder stones. Materials & Methods: 80 patients of urinary stones of both genders were included. Midstream urine specimen was collected from each patient before surgical stone removal and cultured using calibrated (4mm dia.) loop providing fixed quantity on CLED agar, MacConkey agar (MA) and Blood agar plate (BA) (HiMedia Laboratories) for isolation of bacterial pathogens. Cultures were incubated at 37oC for 24 hours. Urinary pH was noted using pH test strip and color change was documented comparing with the provided color chart.