ABSTRACT Giving assignments is one method that can motivate students to learn or train students' level of discipline and psychomotor. This study aims to identify the effect of giving closed and open forms of assignments on the online medium of E-Learning on the level of anxiety experienced by students in the Associate Degree of Midwifery of Megarezky University, Makassar. This study used a Combination Method between research and development methods (Research and Development) with the Experiment Method. About 55 respondents chosen by the sampling technique. The assignment was given twice, including pre-and post-test.At pre-test, the open and closed assignments to develop the product model assignment in the Indonesia Online College application were given to the students. In Post-test, the data collected only using the Google Forms Hars Scale questionnaire instrument. This research was conducted at the Department of Midwifery, Megarezky University, Makassar in November 2020. The data were analyzed using statistical tests using the Mann Whitney test. The study found that the meanvalue of open assignments was 20.93, witha minimum value of 10 and a maximum value of 39. In closed assignments, the mean value was 30.98, with the minimum value was 13 and the maximum value was 48. Based on the Mann Whitney test analysis, the results of giving closed and open onlineassignments through e-learning mediareached a probability value Asymp Sig (2-tailed) 0.000 <0.005. There was a significant influence between giving closed and open forms of assignments on students' anxiety in E-Learning online medium, such as using the Indonesia Kuliah Online application.