act In the present study, a 35 kDa D-mannose specific lectin was purified from the flower extract of Ipomoea pes-caprae (IPL) by lactoferrin-Sepharose 4B in the econo column (BioRad). Physico-chemical analysis revealed that lectin was stable the pH ranges from 6.5-9.5 and temperature ranges from 0°C-60°. The lectin was highly specific for rabbit erythrocytes. UV spectral analysis of lectin documented a characteristic peak at 230 nm and FTIR spectrum showed the IR absorption peaks correspond to amide I and II bands, carboxylic and O-H groups in the lectin. Antimicrobial activity of lectin was tested against four Gram positive bacteria, four Gram negative bacteria and four fungal pathogens. It was observed that the lectin was more effective with a maximum zone of inhibition of 22 mm and 19 mm against B. subtilis andA. flavus respectively.Moderate activities were observed against all the bacteria and fungus tested ranging from 12 mm to 18 mm. The IPL lectin was checked for its antiproliferative and cytotoxicity effect against three cancer cell lines and also with normal fibroblast cell line by MTT assay. The result shows MCF-7 was more sensitive to IPL with lowest IC50 value of 161.01 µg/ml and exhibit low toxicity against normal cells with IC50 value of 374.36 µg/ml. As a result, this research opens up new possibilities for lectin pharmacology.