The current study's objective was to examine any possible antibacterial activity of the biopolymer (Dextran) against clinical isolates of Enterococcus faecalis. All 30 strains of E. faecalis confirmed positive for resistance to vancomycin(VAN), whereas 24 strains (80%) tested positive for resistance to teicoplanin(TEC) in antimicrobial susceptibility testing. The minimum inhibitory concentration (MIC) had been detected by evaluating the antibacterial activity using the broth microdilution technique; the findings revealed that the MIC was between (25-50) mg/ml against isolates of E. faecalis. All experiments were done in triplicate.