To study the anticancer activity of isolated compounds from root of Coccinia grandis whole plant by SRB assay method on DU-145 and PC-3 human prostate cancer cell lines. Materials and methods: Anticancer activity of isolated constituents of Coccinia grandis was performed on SCC-29B and Ishikawa cancer cell lines by the Advanced Centre for Treatment Research and Education in Cancer (ACTREC) Mumbai, India. Cell line had been developed within RPMI 1640 medium that contains 10% fetal bovine serum and 2 mM L-glutamine with the help of SRB assay along with the absorbance had been recorded on an Elisa plate reader at a wavelength of 540 nm with 690 nm. Results: Isolated constituents particularly kampferol showed LC50, TGI and GI50 activity at >80, 69.7 and <10μg/ ml on DU-145 and >80μg/ ml of GI50 activity on PC-3 cell lines. Conclusion: Kampferol from Coccinia grandis has showed potent anticancer activity on DU-145 and PC-3 human prostate cancer cell lines.