Endophytes reside in the tissue of live host plants and help them absorb nutrients, fix nitrogen, defend against diseases, and form symbiotic relationships without endangering the host plant. Secondary metabolites in diverse plants, animals, and microbes have been found to have therapeutic potential as a result of recent inquiry and investigation of these entities for their bioactive potential features. It has been studied how endophytic fungi function as bio factories for new bioactive compounds. An endophytic fungus strain MBKF16 was isolated from edible shoots of Bambusa tulda. The submerged fermentation method was used to mass culture the isolated fungal culture in order to produce extracellular bioactive compounds. Chloroform, methanol, ethyl acetate, and water are the solvents used for the extraction method. The acquired extract was screened for phytochemical analysis. Agar well diffusion assay was performed for antagonistic efficacy against clinical pathogens Salmonella typhi, Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae, which are acquired from KIMS Madikeri. MBKF16 Endophytic fungi are identified molecularly by the 18S rRNA partial gene sequencing method. ITS region obtained are compared with closely related species from the NCBI database. The sequences of MBKF16 are submitted to NCBI with accession number ON287260. A phylogenetic tree is constructed using MEGHA 11 software. Results showed that Rigidoporus vinctus is a potential fungus for the production of numerous bioactive compounds. Ethyl acetate is a highly efficient solvent for extracting active antibacterial compounds, then water, methanol, and chloroform. Extracts confirm the presence of phenols, alkaloids and flavonoids. Ethyl acetate extract has significant antibacterial activity.