Antibiotic toxicity and multi drug resistant pathogens are the two greatest challenges being faced by today's medical world. In the present study, the antimicrobial activity of spices has been investigated as an alternative to antibiotics in order to tackle these dangers. In search of bioactive compound, methanol ethanol and aqueous extract of 3 three Indian spices were screened for antibacterial property. The antibacterial activity of three common Indian spices namely clove, cinnamon and turmeric against bacteria Staphylococcus aureus. The results revealed that the methanol extracts of spices (MIC values of 20- 100 μl/ml) have high antimicrobial activities on all test organisms (range of inhibition, 6- 16 mm) as compare to ethanol and aqueous extracts of spices in same concentration. Results concluded that these spices contain high amount of secondary metabolites due to these metabolites they have high antimicrobial activity and it can be used as good bio- preservater and it can also use for medicinal purpose.