A high nutritious diet along with regular exercise is essential to maintain physical and psychological health. Physical inactivity leads to chronic low grade inflammation and is the leading risk factor for obesity and many other diseases. Few studies revealed that strenuous exercise may also promote systemic low grade inflammation and increase the pro - inflammatory cytokines. The inflammatory cytokines increase the risk of morbidity. Recently much research has been going on natural and synthetic formulations that bring exercise benefits. One such herb with health promoting bio active compounds is Ocimum basilicum. Aim: We aimed to find out the anti-inflammatory and anti-obesity effect of basil seeds supplementation in both non exercisers and high intensity resistance training healthy male individuals. Materials and Methods: The study included 150 healthy male individuals in the age group of21-35 years. The participants were divided into three groups: Non-exercise individuals who consumed basil seeds (Basil seeds group BS, n=50), High intensity resistance training group (HIRT, n=50) and HIRT group along with basil seeds supplementation (BS+HIRT, n=50). These 3 groups underwent 8 weeks of intervention. BS and BS+HIRT participants consumed 10 gm of basil seeds soaked in one litre of water, 6 days/week. High intensity training included 8 weeks with 5 sessions per week. We measured lnterleukin-6 (IL-6), Tumor necrosis factor– α (TNF-α) and leptin levels at the baseline and after 8 weeks of intervention. Blood samples were obtained after overnight fasting. Results: At the baseline mean value of IL-6 is 30.05 pg/ml, TNF –α is 34.07pg/ml and leptin is 8.34ng/ml in all the three groups. There was significant reduction in IL-6 and TNF –α and leptin in BS group after consumption of basil seeds for 8 weeks (23.6±2.19pg/ml, 18.01±1.73pg/ml, 3.77±1.12ng/ml respectively with p<0.001). The HIRT group showed significant elevation in IL-6 (46.85±4.16pg/ml, p<0.001) and TNF–α (81.24±4.14pg/ml, p<0.001) after 8 weeks of resistance training. IL-6 and TNF–α levels were less in BS +HIRT (40.01±4.11 pg/ml, 54.77±5.34 pg/ml respectively; p<0.001) compared to HIRT group. Leptin levels were reduced from 8.35±1.44 ng/ml to 3.77±o.79 ng/ml with p<0.001 in all the three groups after 8 weeks of intervention. Conclusions: Basil seeds supplementation reduced the pro-inflammatory status. The reduction in leptin levels concluded that basil seeds have anti-obesity properties in both non-exercisers and resistance training individuals.