An organization must have a good performance to exist and excel in the competition. The organization that is built certainly has certain goals and to achieve these goals requires the role of human resources. The study was to analyze the implementation of organizational culture. The study was qualitative with a descriptive design. The informants were the Head of Services, Administration, Finance and Programs, Nursing, Installation, Nursing Committee, and Officers using purposive sampling. Data were analyzed by triangulation. The results obtained from several fields studied that the administration sector still lacked organizational involvement, all the fields studied were quite consistent in carrying out their duties and functions and were able to adapt well to the changes in existing regulations, and each field has carried out tasks by the achievement of the mission targets that are already in the hospital. Analytical hierarchy process obtained 62% that the involvement of officers related to human resources was a priority to solve problems in organizational culture. It is hoped that the Dumai City Hospital can improve the quality of resources, especially involving officers in hospital activities to implement an organizational culture by providing training to both government employees and nongovernment employees.