Background: Rotavirus is the leading cause of severe diarrhea among children worldwide. The present study was conducted to assess prevalence of rotavirus diarrhea in know population. Materials & Methods: The study was conducted among children age group 3–36 months who were admitted with acute diarrhea over a period of 1 year. Demographic details were recorded. Rotavirus testing was performed by a laboratory technician with expertise in rotavirus identification. All statistical analyses were carried out using SPSS 17.0 Inc (SPSS, Inc., USA). Statistical significance was set as a p-value < 0.05. Results: In the present study, 400 children aged 3–36 months with an episode of acute diarrhea enrolled in the hospital were evaluated. Out of 400 children, 70% were male and 30% were female child. Maximum children belong to age group 13-24 months. Stool samples of 84 children (21%) were confirmed to have rotaviruses from a total of 400 children