Background: The hematological values can change during and after vigorous exercise, which can vary according to gender, age, environment or nutrition. The present study was conducted to assess High intensity exercise induced changes of hematological profile in sedentary post-pubertal boys and girls. Materials & Methods: The present study 50 sedentary school going post-pubertal boys (n=25) and girls (n=25) belonging to the age group of 15–17 years were included in the study. Estimation of hematological parameters RBC count, hematocrit, Hb concentration, were estimated. Statistical analysis Statistical Package for the Social Sciences (SPSS, v 20.0; Chicago, IL) was used for the statistical analysis of the data. The level of significance was set at P < 0.05