ABSTRACT Background: Supplementation of vitamin D with Oral hypoglycaemics is known to reduce the levels of blood glucose. This study evaluated the role of vitamin D and its combination with metformin on blood glucose level, body weight and assess the effect on serum IL-6. Material and Methods: An experimental study was conducted in Department of Pharmacology and Therapeutics, King George’s Medical University, Lucknow. Total of 30 wistar rats divided into 5 groups with 6 rats in each group. All rats were induced type 2 diabetes after administration of high-fat diet (HFD) for 2 weeks followed by an intraperitoneal (i.p.) injection of single low dose of streptozotocin (STZ) (35mg/Kg) dissolved in cold sodium citrate buffer (pH 4.5, 0.1 M) in a dose volume of 1ml kg−1 except the first group that served as control untreated group. A total of 6 diabetic rats treated with an oral dose of vitamin D3 (500IU/day) with metformin (200 mg/day) for 7 weeks along with HFD comprised the Group 3 of the study while another 6 diabetic rats treated with an oral dose of vitamin D3 (100IU/day) with metformin (200mg/day) for 7 weeks along with HFD comprised Group 4 and 6 diabetic rats treated with metformin (200mg/kg) for 7 weeks along HFD who comprised Group 5 of the study. Results: A total 6 rats were fed on normal pellet diet throughout the experiment. There was a statistically a significant difference in the blood glucose levels in this study (p<0.05). The intergroup comparison also shown the same. The intergroup comparison of IL-6 had also shown a significant difference (p<0.05). The post hoc analysis had also shown a significant difference between the groups. Conclusion: The authors conclude that there is a significant reduction of serum glucose and IL-6 levels in rats treated with the combination of Vitamin D and Metformin than the controls.