Aim: The aim of this ex-vivo stereomicroscopic study was to compare the dissolution efficacy of Xylene, Endosolv-R and Eucalyptol along with H-file in retrieving GuttaPercha and AH Plus sealer from the obturated root canal. Materials and Methods: A total of 30 freshly extracted lower permanent mandibular first premolars were obturated and the samples were placed in incubator to maintain 100% humidity at 37℃ for 7 days. After a week, samples were decoronated to standardize the working length (15mm from apices to coronal end). Reservoir of 3-4 mm was created inside each canal and samples were divided equally into three groups with Xylene (group 1=10), Endosolv (group 2=10) and Eucalyptol (group 3=10). 1 ml of each solvent was dispensed in each tested sample and waited for 2 minutes, retrieval was carried out using 25# H-file. After radiographic verification, longitudinal splitting of each sample was done with mandrel and disc. The images were visualized under stereomicroscope at 20x magnification to access the presence and absence of filling materials along the entire sections, photographs were captured using digital camera and images were transferred to Digimizer 4.5 version for evaluation of the area devoid of obturating materials. The collected datas were assembled for further statistical evaluation using ANOVA and Tukey HSD test. Results: On evaluation, all the tested agents, produced certain degree of dissolution. On comparing the mean values between Xylene, Endosolv-R and Eucalyptol in coronal portion, it was found to be 10.763, 7.143 and 5.94, in middle third the mean obtained was 9.526, 5.408 and 5.906 whereas in apical third the values were 9.334, 6.002 and 6.123 respectively. Conclusions: The results of our study showed statistically higher dissolution capacity with Xylene when compared to Endosolv-R and Eucalyptol, whereas, no significant difference was noted between the dissolving capacity of Endosolv-R and Eucalyptol group. The mean obturation loss in coronal, middle and apical third with Xylene was found to be highest when compared with others. On comparison between Endosolv-R and Eucalyptol, the mean area loss with Endosolv was found to be higher in coronal third, whereas, Eucalyptol was more effective in dissolving the materials in middle and apical third region.