This research paper entitles the research work regarding bio-hydrogen generation from a renewable source, namely biomass; such as a group of photosynthetic microorganisms cyanobacteria. Molecular hydrogen is widely regarded as the energy carrier of the future. It can be used in fuel cells, generating an electric current. Biohydrogen production has several advantages when compared to photoelectrochemical or thermo-chemical processes due to the low energy requirement and investment cost. Cyanobacteria has an inherent ability to produce molecular hydrogen via the enzyme complex nitrogenase. This hydrogen is not released, however but is recaptured by the bacteria using an uptake hydrogenase. The study also foretells about optimization of the various key parameters that enhance hydrogen production. This knowledge can be used to evaluate the possibilities for producing hydrogen and high value products efficiently in the same process.