Abstract: Three types of Iraqi truffles, which were collected from different regions of the Western Desert in Anbar Governorate in western Iraq, were studied. The three types of truffles were diagnosed as alhurqa truffles that are black-dark brown Terfezia boudieri ,and the second type is truffle aljabah that is brown-light-brown in color Terfezia claveryi. The third type was white to creamy color, and brittle solid with zigzags and cracks in its outer shell Tirmania nivea . Models of soil grouped with the three types were studied to determine the chemical and physical properties of the soil, as well as the total numbers of bacteria and fungi present in the truffle rhizosphere were calculated. as well as the total numbers of bacteria and fungi present in the truffle rhizosphere. Amino acids, which differed between the three types, were diagnosed in terms of the quality and quantity of the amino acid, The truffle T. boudieri containing 14 amino acids whose values ranged from 0.011 ppm for Aspartic to 75.654 ppm for Arginine, the total concentration of amino acids was 239.609 ppm While T. claveryi contained 0.192 ppm Aspartic acid to 194.433 ppm Glutamic and the total concentration of amino acids was 346.633 ppm. At last T. nivea content 0.294 ppm Isoleucine to 233.555 ppm Alanine, and the total concentration of amino acids was 556.365 ppm. Soil characteristics varied between the three types of truffles. As well as the amount of nutrients in addition to the difference in the numbers of bacteria and fungi between the truffles types. The truffles oxidant results according to estimate Thiobarbituric acid showed higher value in T. boudieri (0.835) than T. claveryi (0.649) and T. nivea (0.486) mg m Malondialdehyde (MDA). kg truffles.