Stress can be either acute or chronic. Stress is associated with altered hormonal secretions of cortisol. Cortisol is a steroid hormone, which is released during stress. Adaptogens are herbs that improve the responses to stress. In traditional medicine, the Ajaswagandhadhi Lehyam is found to reduce stress induced damage and thereby reduces oxidative stress, facilitating fertility in male Wistar rats because of its radical scavenging activity and also due to the presence of phenolic compounds and flavonoids. This study deals with the adaptogenic activity of Ajaswagandhadhi Lehyam, bringing out the decrease in Cortisol level at different dosage of its administration on Wistar rats.