Abstract: Hepatocyte is the primary functional cell of the liver. More than 80 % of liver is constructed by the hepatocyte cell. Calcium is working as a second messenger. The particular cytosolic Ca2+ level in hepatocyte cell is responsible for frequently working of the liver. In signal transduction calcium play an essential role in hepatocyte cell. Many biological processes are controlled by the calcium like secretion, muscular contraction, cell differentiation, movement of cell, signaling in cell and processes of buffering etc. Diffusion of calcium in the cell is represented by the reaction-diffusion equations. Here twodimensional model is developed to estimate the effect of the essential biophysical parameters as association coefficient, bound buffer, influx and coefficient of diffusion over profiles of calcium on hepatocyte cell. The aim of the study is to analyze the effect of cytosolic calcium diffusion in hepatocyte cell when the buffer is present in large amount. The results have been used to establish the relationships among various biophysical parameters.