One in every four couples in developing countries is affected by infertility. The magnitude of the problem calls for urgent action, particularly when the majority of cases of infertility are avoidable. Of 60-80 million couples suffering from infertility every year worldwide, probably between 15 and 20 million (25%) are in India alone. A case-control study layout was adopted for the present study on relation between hormonal parameters and risk markers in infertility the test subjects were referred from various infertility clinics were chosen for the study. Out of 150 study subjects, 100 infertile women showed increased FSH concentration and they revealed increased MDA concentration, mean b/c value and mCBMNF. Infertile women with low concentration of estradiol demonstrated an elevated MDA concentration, mCBMNF and mean b/c value than others with increased level of estradiol. Out of 150 study subjects, 115 individuals showed an increased PRL concentration and they revealed increased values of MDA concentration, mCBMNF and mean b/c value.