Ramnad white is an important indigenous sheep breed in Tamil Nadu and has a decreasing trend in population, which is alarming as the breed is in critically endangered. Artificial insemination is needed for frequent breeding and for the conservation of these animals. Further, the artificial insemination is also difficult in sheep because of few external estrus signs. In mammals, detection of estrus is one of the most critical components for successful breeding. The accurate and consistent detection of estrus is also necessary for successful fertilization and increase the percentage of success in artificial insemination. Normally, reproduction depends upon the odours released from the female during the estrus phase. It is well known that mammals emit chemo-signals to the surroundings via., faeces, saliva, urine and specialized scent glands. Analyzing chemical signals in the faeces of Ramnad white will aid us to develop estrus detection kit for easy detection of estrus, so that we can proceed for the improvement of livestock production. Therefore, the prime objective of the present work is to identify the marker for ovulatory phase in females and to develop a kit, which would enable to easily identify the reproductive stage of the animal and allow it for mating and artificial insemination. On the other hand, chemical signals (pheromones) would be used for provoking sexual behavior in males. The present work has been designed to analyze the volatiles present in faeces across the various estrous phases in order to identify estrus markers in female sheep and a behavioral analysis are also used as supporting information for our present findings. The samples were collected from sheep in different stages of estrous viz. proestrus, estrus and postestrus. They were extracted using dichloro methane for GC-MS analysis. The results showed the presence of nine different compounds with varying molecular weight and retention time. Among the identified compounds, β-caryophyllene has been exclusively present only in estrus stage and absent in all other stages. The present study concluded that the estrus specific compound β-caryophyllene may act as a pheromonal compound involved in ram communication.