Abstract Cotton, often known as "The White Gold" or "The King of Fibres," is the most important cash crop in the nation since it is used in the booming textile sector. About sixty million people depend on it for their survival, and it's a valuable agricultural product that helps millions of farmers in both rich and developing nations make a living. The fungus Alternaria alternata (Fr.) Keissler causes a devastating leaf spot on Bt cotton plants, while the bacterium Xanthomonas citripv. malvacearum (Smith) Dye causes a bacterial blight. Alternaria leaf spot and bacterial blight infections were the most frequent and both are major problems in Gujarat that have particular significance in the current study since they lead to economic yield losses in Gujarat. Corynesporacassiicola, the causal agent of a new and peculiar leaf mark, has been discovered.