Background: Studies have shown that patients with type 2 diabetes mellitus(T2DM) often have lower serum magnesium levels compared to healthy individuals. Magnesium is an essential mineral that plays a key role in several metabolic processes, including glucose metabolism, insulin secretion and insulin action. Aim and objectives: To compare the level of serum magnesium in type 2 diabetes patients and non diabetic healthy controls. Materials and Methods: A Case-Control study was conducted in 50 diabetic patients and 50 non- diabetic healthy controls who attended General Medicine OPD at Chalmeda Anand Rao Institute of Medical Sciences, after following inclusion and exclusion criteria and also after approval of institutional ethical committee. Results: Total 100 patients divided in to each 50 cases and controls, demographic profile of the study population. Showed that there was not statistically significant difference between age and gender in cases and controls. Mean magnesium level was statistically highly significant between cases and controls and its observed less among cases compared to controls. (P-value<0.001). Conclusion: Serum magnesium levels were lower in type 2 diabetes patients when compared to healthy non-diabetic controls.